Operating system: Windows-10-10.0.17763 64 Bits
Graphics card: Intel(R) UHD Graphics 620 Intel 4.5.0 - Build 18.104.22.16800
Broken: version: 2.81 (sub 3), branch: master, commit date: 2019-08-20 22:35, hash: rBdaba3e871ff5
Short description of error
Blender cannot save the two-column layout of toolbar in the 3D Viewport
Exact steps for others to reproduce the error
When we start Blender, the toolbars appear in single column.
That needs too much scrolling. So I prefer two-column toolbar layout.
But the problem is that every time I start Blender, it goes back to the default single-column layout.
I saved the default startup file and also user preferences (after changing the layout of the toolbar).
(I have no idea which of these two steps would save the toolbar layout, so I tried both.)
But both actions had no effect.